Jun Moriwaki, Michito Shimozuru, Hifumi Tsuruga, Tsutomu Mano, Toshio Tsubota . Estimation of reproductive parameters and their annual variation in brown bears of Hokkaido, Japan2010 · 記述言語: 英語 掲載種別: 研究論文(学術雑誌) 出版者・発行元: INT ASSOC BEAR RESEARCH & MANAGEMENT-IBA The reproductive tracts of 130 adult female brown bears (Ursus arctos) in Hokkaido, Japan, euthanized during 2010-2013 for conflict management, were examined for the presence and number of corpora lutea (CL) in the ovary and placental scars (PS) in the uterine horns. We scored PS on a 0-5 scale based on morphological features including their rim and color. They were characterized as representing either current-year parturition, as determined by accompanying cubs, or previous year parturition, as determined by accompanying yearlings. In total, 128 females were divided into 3 reproductive categories: 44 females with current-year parturition, 39 with previous-year parturition, and 45 solitary females that had not given birth for >2 years. The estimated ovulation rate was 2.21 +/- 0.55 (mean +/- SD), the implantation rate was 1.91 +/- 0.74, and the litter size was 1.54 +/- 0.51. Implantation rates were lower than ovulation rates and changed annually during 2010-2013. Systematic observation of PS and CL allowed us to evaluate the reproductive history and parameters in Hokkaido brown bears and ascertain their annual variation. DOI: 10.2192/URSU-D-16-00005.1 Web of Science
Yuri Shirane, Michito Shimozuru, Masami Yamanaka, Hifumi Tsuruga, Saiko Hirano, Natsuo Nagano, Jun Moriwaki, Masanao Nakanishi, Tsuyoshi Ishinazaka, Takane Nose, Shinsuke Kasai, Masataka Shirayanagi,1998 · 記述言語: 英語 掲載種別: 研究論文(学術雑誌) 出版者・発行元: SPRINGER Understanding natal dispersal patterns is fundamental in the ecology and conservation biology of large wild carnivores. In this study, we used two approaches to determine genetic variation and dispersal patterns of brown bears in the Shiretoko Peninsula, eastern Hokkaido, Japan. The first approach was a large-scale genetic analysis. We analyzed haplotypes from the mitochondrial DNA (mtDNA) control region of 760 individual samples collected throughout the peninsula during 1998-2016. We detected seven haplotypes, including two that were confirmed for the first time. In females, the distribution of haplotypes was geographically structured, whereas haplotypes in males were distributed widely throughout the peninsula. Only some males in the lower peninsula had haplotypes that were not detected within the peninsula. The second approach was a local-scale genetic analysis, including intensive focal sampling in the Rusha area, a special wildlife protection area on the peninsula. Proportions of mtDNA haplotypes in adult bears were investigated and compared between the sexes. Although more than half of the females had the same haplotype, males had more diverse haplotypes, suggesting that they came to the Rusha area from other regions. Thus, our study revealed that mtDNA haplotype distribution has been maintained by female philopatry, and that bears exhibit male-biased dispersal. Furthermore, the lower peninsula appears to act as a contact zone between the peninsula and mainland Hokkaido, which is important for maintaining genetic diversity. DOI: 10.1007/s10344-018-1222-x Web of Science