Fukugami S., Yamasaki M., Kokushi E., Uno S. . Influence of CYP1A and AhR modulation on polycyclic aromatic hydrocarbon-induced developmental defects in Japanese medaka . Aquatic Toxic2025 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Aquatic Toxicology Polycyclic aromatic hydrocarbons (PAHs) are known to induce developmental malformations in fish embryos. However, the interaction between aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) in PAH-induced development defects remains unclear. Therefore, we investigated the effects of the CYP1A inhibitor piperonylbutoxide (PBO) and the AhR antagonist CH223191 (CH) on the development of Japanese medaka (Oryzias Latipes) embryos exposed to different PAHs. Japanese medaka embryos were exposed to three conditions: PAH alone, PAH and PBO, and PAH and CH. Microscopic observations were performed to examine the presence of developmental defects. Although neither phenanthrene (Phe) nor fluoranthene (Flu) induced morphological malformations in larvae, benzo(a)anthracene (BaA) exposure induced craniofacial deformities in the larvae. Additionally, BaA and PBO co-exposure significantly increased the rate and severity of malformations. Pyrene (Pyr) exposure induced craniofacial defects, cardiac hypertrophy, pericardial edema, and spinal curvature, which were attenuated by exposure to either CH or PBO. Collectively, these findings suggest that structurally different PAHs exert their toxic effects via distinct mechanisms during fish development. DOI： 10.1016/j.aquatox.2025.107267 Scopus PubMed
Seki T., Katsura M., Yamasaki M., Yamashita K., Kokushi E., Uno S. . Effects of diphenhydramine exposure on reproduction of mature Japanese medaka (Oryzias latipes) . Chemosphere3582024 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Chemosphere Diphenhydramine (DPH) is an antihistamine drug. It has been frequently detected in the environment, because it is not completely degraded in wastewater treatment plants. Recent studies have shown the adverse effects of DPH exposure to various aquatic organisms; however, its chronic effects on fish have been poorly elucidated. In this study, several pairs of mature Japanese medaka (Oryzias latipes) were exposed to DPH for a long period to determine the effects of DPH exposure on the subsequent generations, number of spawned and fertilized eggs, expression of sex-related genes, feeding behavior, embryo development, hatching rate, malformations among the hatched larvae, and mortality rate. The number of spawned eggs significantly decreased, when the parent fish were continuously exposed to 31.6 μg/L DPH for over 46 days. DPH exposure also altered the feeding behavior of medaka individuals, and increased the larval mortality rate. The effects of DPH exposure to fish may occur to some extent in the actual aquatic environment, although the risk evaluations in the field are limited. DOI： 10.1016/j.chemosphere.2024.142163 Scopus PubMed
