Tateo S., Shinchi H., Matsumoto H., Nagata N., Hashimoto M., Wakao M., Suda Y. . Optimized immobilization of single chain variable fragment antibody onto non-toxic fluorescent nanoparticles fo2023 · 記述言語： 日本語 出版者・発行元： Colloids and Surfaces B: Biointerfaces Single-chain variable fragment antibody (scFv) is a small molecular weight antibody that can be used for both therapeutic and diagnostic purposes. To visualize the interaction with the target biomolecules, scFv must be labeled with fluorescent molecules. In this study, to achieve the efficient labeling of scFv, we developed scFv-fluorescent nanoparticle conjugates to utilize scFv as bioprobes. As fluorescent carriers, cadmium-free ZnS-AgInS2/ZnS core/shell nanoparticles were used, and scFv was immobilized onto the nanoparticles via the interaction of nickel ions on nitrilotriacetic acid and hexahistidine (His-tag) fused with scFv. UV-Vis, fluorescence spectra, NMR, and dynamic laser scattering were used to characterize the scFv immobilized fluorescent nanoparticles (scFv-FNPs). The amounts of scFv on FNPs were controlled by the concentration of scFv. The scFv-FNPs that were prepared were non-toxic and selectively bound to cancer cells. The scFv-FNPs could be used as bioanalytical tools, and the immobilization method described here is a promising method for labeling biomolecules with the His-tag. DOI： 10.1016/j.colsurfb.2023.113192 Scopus PubMed
Kucho K.I., Hashimoto M., Normand P., Fournier P., Pujic P. . Structure of surface polysaccharides affects symbiotic nitrogen fixation in Frankia alni ACN14a . Journal of Forest Resear2022 · 記述言語： 日本語 出版者・発行元： Journal of Forest Research Frankia spp. are nitrogen-fixing actinobacteria that establish root-nodule symbiosis with actinorhizal plants comprising more than 200 species in eight dicotyledonous families. From a cell population of Frankia alni strain ACN14a that had been subcultured for over 30 years, we identified two types of variants (types A and S) with a different colony appearance. Type A variants exhibited ambiguous, and type S variants exhibited sharp colony edges. The two types differed in molecular weight and monosaccharide composition of cell surface polysaccharides, which could be responsible for the differences in colony appearance. When inoculated to a host plant Alnus glutinosa, both types induced nodulation but plants infected with type S isolates showed much lower nitrogen fixation activity than those infected with type A isolates. Indeed, type S isolates developed fewer vesicles inside infected plant cells. These results suggest that surface polysaccharides of Frankia play important roles in symbiotic interactions with actinorhizal plants. DOI： 10.1080/13416979.2022.2136561 Scopus
