Kawashima Y., Hagimoto A., Abe H., Miyakoshi M., Kawabata Y., Indo H., Tanaka T. . Using texture analysis of ultrasonography images of neck lymph nodes to differentiate metastasis to non-metas2024 · 記述言語： 日本語 出版者・発行元： Journal of Oral and Maxillofacial Surgery Medicine and Pathology Object: To differentiate between metastatic neck nodes and non-metastatic neck nodes in oral maxillary gingival squamous cell carcinoma, textural analysis of these lymph nodes in ultrasound images was performed in this study. Methods: Twenty five metastatic neck nodes and 28 non-metastatic neck nodes were enrolled in this study. Seventy eight texture characteristics were retrieved from the US images using the LIFEx software. The Mann Whitney U test was measurably utilized to survey on the off chance that there was a measurably noteworthy distinction within the textural characteristics between metastatic neck nodes and non-metastatic neck nodes. The capacity of the surface highlights to recognize between metastatic neck nodes and non-metastatic neck nodes was illustrated utilizing the Receiver Operating Characteristic analysis curves (ROC). Youden's J statistic was used to determine the cut-off positions in each ROC curve that maximized sensitivity and specificity. Results: Zone size non uniformity (ZSNU) highlight appeared the foremost noteworthy contrast between these nodes (p < 0.001). Strength had Area Under the Curve (AUC) of 0.811, specificity of 0.821 and sensitivity of 0.8, when measured at the cutoff value of 896.344. Conclusions: Our results come about uncovered that quality highlight may be the finest surface highlight to distinguish from non-metastatic neck nodes and to anticipate metastatic neck nodes in oral maxillary gingival squamous cell carcinoma. DOI： 10.1016/j.ajoms.2024.07.013 Scopus
Cornette R., Indo H.P., Iwata K.i., Hagiwara-Komoda Y., Nakahara Y., Gusev O., Kikawada T., Okuda T., Majima H.J. . Oxidative stress is an essential factor for the induction of anhydrobiosis i2023 · 記述言語： 日本語 出版者・発行元： Mitochondrion The sleeping chironomid (Polypedilum vanderplanki) is the only insect capable of surviving complete desiccation in an ametabolic state called anhydrobiosis. Here, we focused on the role of oxidative stress and we observed the production of reactive oxygen species (ROS) in desiccating larvae and in those exposed to salinity stress. Oxidative stress occurs to some extent in desiccating larvae, inducing carbonylation of proteins. Oxidative stress overcomes the antioxidant defenses of the larvae during the first hour following rehydration of anhydrobiotic larvae. It facilitates the oxidation of DNA and cell membrane lipids; however, these damages are quickly repaired after a few hours. In addition to its deleterious effects, we demonstrated that artificial exposure to oxidative stress could induce a response similar to desiccation stress, at the transcriptome and protein levels. Furthermore, the response of anhydrobiosis-related genes to desiccation and salinity stress was inhibited by antioxidant treatment. Thus, we conclude that oxidative stress is an essential trigger for inducing the expression of protective genes during the onset of anhydrobiosis in desiccating of P. vanderplanki larvae. DOI： 10.1016/j.mito.2023.11.002 Scopus PubMed
Tomita K., Indo H.P., Sato T., Tangpong J., Majima H.J. . Development of a sensitive double TaqMan Probe-based qPCR Angle-Degree method to detect mutation frequencies . Mitochondrion702023 · 記述言語： 日本語 出版者・発行元： Mitochondrion We designed a method to examine the mutation frequencies of the A3243G mutation of mitochondrial DNA (mtDNA) in patients with mitochondrial encephalopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome. We performed a qPCR assay using the FAM and VIC TaqMan probes, which detect the 3243G (mutated) and 3243A (wild-type) sequences of mtDNA, respectively. The results obtained by “degree” in a series of differential mutation frequencies were used to plot a standard curve of the mutation frequency. The standard curve was then applied for qPCR assays of the desired samples. The standard deviation (%) of the samples calculated using the standard curve for the TaqMan probe was 2.4 ± 1.5%. This method could be used to examine mutation frequencies in the context of diabetes, aging, cancer, and neurodegenerative diseases. DOI： 10.1016/j.mito.2023.02.010 Scopus PubMed
