Fujino K, Imai H, Matsuya S, Kusakabe KT, Kano K . Total cell number influences the timing of Hippo/YAP activation during preimplantation development in mouse tetraploid embryos. . Bio2025 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Biochemical and Biophysical Research Communications Total cell number may determine the timing of Hippo/YAP activity during first lineage specification. We combined mouse tetraploid embryos (reduced cell numbers) with a 2 × 4n aggregation approach (increasing cell numbers) to test whether YAP nuclear entry in inner cell mass (ICM) cells is temporally shifted. Tetraploids reached the blastocyst stage with approximately half the cells of diploids and exhibited delayed nuclear YAP in ICM at mid-blastocyst, despite overall ICM/trophectoderm patterning and pluripotency markers (Oct4, Nanog, Sox2) being comparable. Increasing total cell number by aggregating two tetraploids restored YAP timing to that of diploids. By the late-blastocyst stage, most tetraploid ICM cells displayed nuclear YAP, indicating that this delay is transient. These findings support a model in which total cell number —not ploidy per se—is associated with the timing of Hippo/YAP activation in the preimplantation embryo. DOI： 10.1016/j.bbrc.2025.152811 Scopus PubMed researchmap
Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K . Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors.1995 · 担当区分： 筆頭著者 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Scientific Reports Mus minutoides is one of the smallest mammals worldwide; however, the regulatory mechanisms underlying its dwarfism have not been examined. Therefore, we aimed to establish M. minutoides induced pluripotent stem cells (iPSCs) using the PiggyBac transposon system for applications in developmental engineering. The established M. minutoides iPSCs were found to express pluripotency markers and could differentiate into neurons. Based on in vitro differentiation analysis, M. minutoides iPSCs formed embryoid bodies expressing marker genes in all three germ layers. Moreover, according to the in vivo analysis, these cells contributed to the formation of teratoma and development of chimeric mice with Mus musculus. Overall, the M. minutoides iPSCs generated in this study possess properties that are comparable to or closely resemble those of naïve pluripotent stem cells (PSCs). These findings suggest these iPSCs have potential utility in various analytical applications, including methods for blastocyst completion. DOI： 10.1038/s41598-024-53687-9 Scopus PubMed researchmap
Matsuya S, Imai H, Iwamori N, Fujino K, Sakurai Y, Yoshimura T, Watanabe K, Fujii W, Mizukami Y, Tsujio M, Kusakabe KT, Kano K . Induction of pluripotency in large Japanese field mouse (Apodem1972 · 担当区分： 筆頭著者, 責任著者 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： 公益社団法人 日本獣医学会 The large Japanese field mouse, Apodemus speciosus, is a rodent species endemic to Japan and is widely distributed throughout the country. It has been extensively used as a model organism in studies of geographic isolation and as an environmental bioindicator. However, there have been no reports on the establishment of pluripotent stem cells from this species that could be applied to developmental engineering. In this study, we attempted to generate induced pluripotent stem cells (iPSCs) from A. speciosus and successfully obtained cells that formed dome-shaped colonies and expressed pluripotency markers. These cells were capable of forming embryoid bodies that expressed markers of the three germ layers, and in vivo analysis using immunodeficient mice confirmed their ability to form teratomas. A. speciosus iPSCs established in this study not only contribute to the conservation of Japan’s valuable animal genetic resources, but also provide a novel platform for investigating various biological processes that remain unexplored in this wild species. DOI： 10.1292/jvms.25-0390 Scopus PubMed CiNii Research researchmap
