京都大学 · 農学部・農学研究科 · 特定准教授

研究关键词biocatalysis・amino acid hydroxylases・recombinant Escherichia coli・amide bond formation・NRPS modules・hydroxyaspartic acid

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• Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-prolineBiochemical and Biophysical Research Communications,
• A high-throughput and generic assay method for the determination of substrate specificities of thermophilic α-aminotransferasesJournal of Microbiological Methods,
• Identification of tryptophanase from Escherichia coli for the synthesis of S-allyl-l-cysteine and related S-substituted cysteine derivativesJournal of Bioscience and Bioengineering,
• Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1Applied Microbiology and Biotechnology,

• 生理活性脂肪酸の安定性と機能性向上を目的とした微生物由来アミド化触媒の探索
• タンデム酵素反応による(+)-アリイン生産バイオプロセスの実用化検討と応用展開
• 微生物酵素を活用した機能性含硫アミノ酸生産プラットフォームの構築
• β-グルコシド配糖体合成に向けた新規糖転移酵素の探索と酵素の機能解析

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