Fukui Prefectural University · Department of Bioresources / Graduate School of Bioresources · 教授

Research keywordsX線結晶構造解析・酵素・タンパク質・応用微生物学・応用生物化学・構造生物化学

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• 1. Itoh, T.: Structures and functions of carbohydrate-active enzymes of chitinolytic bacteria Paenibacillus sp. str. FPU-7.2021 · 2. 伊藤貴文, 日び隆雄, 木元久: 細菌 P. FPU-7によるキチン分解機構と細胞表層提示型キチナーゼ (Chitin degradation mechanism and cell-surface-expressed chitinase of Paenibacillus sp. str. FPU-7) アグリバイオ 2018年12月臨時増刊号, 2018.
• 【原 著 論 文】2021 · 2. Itoh, T., Kamiya, A., Kimura, M. and Murai, K.*: Heterogeneous expression and purification of the wheat VRN1 K-box domain suggest the formation of a tetramer of the VRN1 protein. Am. J. Plant Sci. 12, 1002-1010, 2021.
• 3. Panti, N., Cherdvorapong, V., Itoh, T., Hibi, T., Suyotha, W., Yano, S. and Wakayama, M.*: Functional analysis of α-1,3-glucanase domain structure from Streptomyces thermodiastaticus HF3-3. J. Gen. Appl. Microbiol. 67, 85-91, 2021.2021 · 5. Sano, C., Itoh, T., Phumsombat, P., Hayashi, J., Wakayama, M. * and Hibi, T. *: Mutagenesis and structure-based analysis of the role of Tryptophan525 of γ-glutamyltranspeptidase from Pseudomonas nitroreducens. Biochem. Biophys. Res. Commun. 534, 286-291, 2020.
• 6. Phumsombat, P., Sano, C., Ikezoe, H., Hayashi, J., Itoh, T., Hibi, T. and Wakayama, M.*: Efficient production of L-theanine using immobilized recombinant Escherichia coli cells expressing a modified γ-glutamyltranspeptidase gene from Pseudomonas nitroreducens. Adv. Biol. Chem.10, 157-171, 2020.2020 · 8. Iwasaki, Y., * Itoh, T., Hagi, Y., Matuta, S., Nishiyama, A., Chaya, G., Koboyashi, Y., Miura, K. and Komatsu, S. *: Proteomics analysis of plasma membrane fractions of the root, leaf, and flower of rice. Int. J. Mol. Sci. 21: E6988, 2020.

• 細胞表層提示型キチナーゼのキチン分解機構の解明と糖転移活性向上によるオリゴ糖生産
• Paenibacillus属細菌によるキチン利用機構の解明
• ループデザインによる耐熱性好冷酵素の創出
• 細菌P. FPU-7によるキチン分解機構の解明と細胞表層タンパク質提示の利用

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