Kagoshima University · Graduate School of Medical and Dental Sciences

Research keywordschorea-acanthocytosis・McLeod syndrome・VPS13A mutations・XK mutations・repeat expansion epilepsy・ferroptosis

This is a public-data preview. Personalized fit, contact angles, and saved workflows require sign-in.

• Akane Terasaki, Masayuki Nakamura, Yuka Urata, Hanae Hiwatashi, Izumi Yokoyama, Takeshi Yasuda, Teiichi Onuma, Kazumaru Wada, Sunao Kaneko, Rumiko Kan, Shin-ichi Niwa, Ohiko Hashimoto, Osamu Komure, Yu-ichi Goto, Yuko Yamagishi, Misa Nakano, Yoshihiko Furusawa & Akira Sano2020 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌）
• Nishida Y. . Novel pathogenic VPS13A gene mutations in Japanese patients with chorea-acanthocytosis . Neurology: Genetics5 ( 3 ) e332 2019年62019 · 出版者・発行元： Neurology: Genetics DOI： 10.1212/NXG.0000000000000332 Scopus PubMed
• Nishizawa Y., Sakimoto H., Nagata O., Sasaki N., Urata Y., Arai K., Hiwatashi H., Yokoyama I., Kishida S., Sano A., Nakamura M. . Chorein deficiency promotes ferroptosis . FEBS Open Bi記述言語： 日本語 出版者・発行元： FEBS Open Bio Ferroptosis is a type of programmed cell death owed to an intracellular accumulation of iron resulting in the generation reactive oxygen species, which in turn can cause peroxidation of plasma membrane lipids and ultimately result in cell death. We investigated the potential involvement of VPS13A deficiency in ferroptosis. The VPS13A gene encodes for chorein, and its deficiency is a molecular cause of chorea-acanthocytosis (ChAc), a Huntington-like disease with neurodegeneration in the striatum. In our previous study, we found male infertility characterized by increased malondialdehyde staining of the spermatozoa in the testes of the ChAc model mice. Thus, in this study we performed metabolome analysis of sperm extracted from the epididymis of the ChAc model mice, which revealed decreased cystine levels, suggesting an association between chorein deficiency and ferroptosis. We then investigated the role of chorein in ferroptosis using VPS13A knockdown (VPS13A-KD) HEK293 cells. We found that VPS13A-KD cells displayed a significantly diminished resistance to tert-Butyl hydroperoxide (tBHP)-induced lipid peroxidation and cell death compared to control cells, which could be rescued by treatment with ferrostatin-1. Moreover, VPS13A-KD cells showed Fe(II) accumulation, suggesting an impaired capacity for divalent iron removal. In the cytosolic fraction of VPS13A-KD cells, the protein level of glutathione peroxidase 4 (GPX4) was significantly reduced, suggesting that dysfunction of chorein impairs GPX4 transport, thereby facilitating ferroptosis. These results suggest that ferroptosis may contribute to neurodegeneration in ChAc caused by loss of chorein function. DOI： 10.1002/2211-5463.13870 Scopus PubMed
• Akane Terasaki, Masayuki Nakamura, Yuka Urata, Hanae Hiwatashi, Izumi Yokoyama, Takeshi Yasuda, Teiichi Onuma, Kazumaru Wada, Sunao Kaneko, Rumiko Kan, Shin-ichi Niwa, Ohiko Hashimoto, Osamu Komure, Y記述言語： 英語 掲載種別： 研究論文（学術雑誌）

• Official profile