Kagoshima University · Division of Fisheries Sciences, School of Agriculture, Fisheries and Veterinary Medicine

Research keywordsFood・science・Animal・Pagrus・major・Soybean・meal・Fermentation

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• Shadrack R.S., Manabu I., Koshio S., Yokoyama S., Zhang Y., Mzengereza K., El Basuini M.F., Dawood M.A.O. . Effects of Single and Mixture Probiotic Supplements on Growth, Digestive Activity, A2022 · 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Aquaculture Nutrition A 50-day feeding trial was conducted to evaluate the efficiency of single and mixed strains of probiotic bacteria supplements on juvenile red sea bream (Pagrus major). The study investigated the growth, digestibility, hemato-biochemistry, antioxidant, immune, immune, and growth gene expression and stress responses of P. major. Three hundred juvenile P. major (21:56 ± 0:09 g) were randomly distributed into fifteen 200-L polyethylene tanks (20 fish per tank) in triplicate of 5 treatments designated as D1 = the basal diet; Streptococcus faecalis (SF) T − 110 5 × 106 cfu/g diet and Bacillus amyloliquefaciens (BA) TOA 5001 5 × 105 cfu/g diet in the second group (D2) at 0.2%; mix SF T − 110 1 × 106 cfu/g diet, Lactobacillus plantarum (LP) TO − A 4 × 105 cfu/g diet, Bacillus mesentericus (BM) TO − A 1 × 102 cfu/g diet, BA TOA5001 5 × 105 cfu/g diet in the third group (D3) at 1%; mix SF T − 110 1 × 106 cfu/g diet, LP TO − A 4 × 105 cfu/g diet, and BM TO − A 1 × 102 cfu/g diet in fourth group (D4) at 0.5%; and single strain BA TOA5001 5×105 cfu/g diet in the fifth group (D5) at 0.5% of dietary proportion. Results showed that D2 and D3 fish groups exhibit better performance, followed by D4, D5, and control group D1. This finding demonstrated that the use of BA in mix strain probiotic bacteria diet (D2, D3) had improved immune response, antioxidant enzymes, immunity (TNF-a, IL-1b), and growth-related (IGF-1, IGF-2) mRNA expression of juvenile P. major compared to the mix strain D4, single strain D5, and the control D1. Furthermore, single strain D5 and mix strain D4 also exhibit relatively better immune responses in P. major than in control D1. Considering the overall fish performances, mix strain SF+BA (D2) and mix strain SF+BM+LP+BA (D3) were the recommended potential mix strain probiotic bacteria supplement for P. major and may be useful also for other related aquatic species. DOI： 10.1155/2022/8968494 Scopus researchmap
• Shadrack R.S., Manabu I., Yokoyama S., Koshio S., Miguel V.A., Yukun Z., Mzengereza K., Seo S., Dossou S., El Basuini M.F. . Specific importance of low level dietary supplementation of Lypomyc2022 · 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Annals of Animal Science Most probiotic yeast supplement in fish exhibit beneficial effect at ≤1% of the dietary proportion. This study aimed at evaluating the specific effects of Lypomyces starkeyi CB1807 yeast strain supplemented at ≤1% of dietary proportion on the performance of juvenile red sea bream (Pagrus major, 1.9 ± 0.04 g). Five diets were supplemented with yeast at graded levels of 0% (control diet 'CD1'), 0.05% (D2), 0.1% (D3), 0.5% (D4), and 1.0% (D5). After 45 days of feeding trial, significant (P<0.05) improvement was detected on final body weight (FBW) and body weight gain (BWG) in fish fed D3 and D5 compared to control. Low values of total cholesterol (T-Cho) and aspartate aminotransferase (AST) were recorded in fish groups fed on D2, D4, and D5, respectively. Fish fed on D3, D4 and D5 diets showed high (P<0.05) values of serum, mucus and liver lysozyme compared to control. Fish fed on D5 showed high values of total immunoglobulin (Ig) compared to control. Fish fed on D2 showed strong correlation with biological antioxidant activity (BAP), superoxide dismutase (SOD) and catalase activity (CAT). The biological antioxidant potential (BAP) activity in fish fed on D2 was significantly higher compared to control (P<0.05). The reactive oxygen metabolites (d-ROM) were significantly lower in fish fed on D2 and D3 compared to CD1 (P<0.05). Peroxidase activity was improved significantly (P<0.05) in fish fed on D3, D4 and D5 compared to control. The tolerance ability (LT50) of fish fed on D5 against low salinity stress was significantly higher compared to control (P<0.05). It was concluded that dietary benefits of spent L. starkeyi yeast at ≤1% showed considerable improvement in antioxidant capacity in red sea bream, P. major. DOI： 10.2478/aoas-2022-0012 Scopus researchmap
• Mzengereza K., Ishikawa M., Koshio S., Shadrack R.S., Zhang Y., Dossou S., Kotani T., Shahin S.A., Zaineldin A.I., Waqalevu V., Dawood M.A.O., Hassan A.M., Al-Sharif M.M., El Basuini M.F. . Re2022 · 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Aquaculture Reports The current work assessed the inclusion impacts of oilseed camelina meal (CM) as a protein source in red sea bream (Pagrus major) diets. A 45 day assessment period with 180 juveniles (6.47 ± 0.17 g) were allocated in triplicates to 4 experimental groups and fed formulated diets in which fish meal (FM) was subrogated at graded series of 0% (T1), 20.5% soybean meal (T2), 20.5% camelina meal (T3), and 33% camelina meal (T4). No noticeable alterations were observed in specific growth rate, feed intake, survival rate, hepatosomatic index, and Fulton's condition factor among the experimental groups. Fish fed the T4 diet showed considerably reduced (P < 0.05) final weight and body protein content when compared to those fed T2, T3, and basal diets. Plasma biochemical parameters show no differences (P > 0.05) in glucose, total bilirubin, total protein, total cholesterol, triglyceride, glutamyl oxaloacetic transaminase, and glutamic pyruvate transaminase. Hematocrit levels decreased noticeably (P < 0.05) in fish groups fed camelina meal in comparison to the control group. Liver and intestinal histology showed a healthy status and an increase in villus length and goblet cell number in camelina groups. Fish fed the T4 diet displayed higher expression levels (P < 0.05) of relative mRNA interleukin 1 beta (IL-1b) and tumor necrosis factor-alpha (TNF-α) in comparison to other groups. The inclusion of camelina meal (up to 20%) in red sea bream diets produced similar outcomes to specimens fed fish meal and soybean meal. DOI： 10.1016/j.aqrep.2022.101175 Scopus researchmap
• Zaineldin A.I., Hegazi S., Koshio S., Ishikawa M., Dawood M.A.O., Dossou S., Yukun Z., Mzengereza K. . Singular effects of Bacillus subtilis C-3102 or Saccharomyces cerevisiae type 1 on the gr2020 · 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Annals of Animal Science The beneficial effects of Bacillus subtilis C-3102 and Saccharomyces cerevisiae type 1 were tested in red sea bream (Pagrus major) feeds. A basal diet (control) and two other diets were prepared by supplementation with B. subtilis C-3102 (PB) or S. cerevisiae type 1 (PY). After 60 days, both probiotic-supplemented groups exhibited significant enhancement in growth performance, the protein efficiency ratio (PER), and digestive enzyme secretion (protease and amylase) compared to the control group (P<0.05). The anterior, middle, and posterior parts of the intestines exhibited significantly increased values of intestinal fold height (hF), enterocyte height (hE), and microvillus height (hMV) in fish fed PB-or PY-supplemented diets (P<0.05). Serum peroxidase, anti-protease, and bactericidal activities were enhanced significantly in both probiotic-treated groups compared to the control group (P<0.05). Serum and mucus lysozyme activities improved significantly in the PB group compared to the control group (P<0.05). Catalase activity was also significantly decreased in both probiotic groups, with relatively lower activity observed in the PY group (P<0.05). Both probiotic groups showed considerably increased tolerance to freshwater exposure (P<0.05). In conclusion, B. subtilis C-3102 and S. cerevisiae type 1 can be used as functional probiotics to enhance the growth performance, digestion capacity, gut morphology, immune response, and stress resistance of the red sea bream with relatively higher efficiency by B. subtilis C-3102. DOI： 10.2478/aoas-2020-0075 Scopus researchmap

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