Kagoshima University · Division of Veterinary Medicine, School of Agriculture, Fisheries and Veterinary Medicine · 教授

Research keywordsマイクロRNA・ウシ・エクソソーム・エピジェネティクス・トランスクリプトミクス・メタボロミクス・家畜生理・筋線維型・食肉・骨格筋

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• Koichi Ojima, Mika Oe, Susumu Muroya . Differential expression of miRNAs in slow and fast muscle fibers isolated from GFP-Myh7 mice. . Physiological genomics58 (2025 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） MicroRNAs (miRNAs) are short noncoding RNAs that regulate gene expression in various cell types. Skeletal muscle consists of bundles of muscle fibers, which are classified as either slow-type or fast-type according to their properties. However, the roles of miRNAs in modulating physiological muscle phenotypes remain unclear. Here, we profiled fiber-type-enriched miRNAs to gain insight into differences in gene regulation between the two fiber types. To avoid cross-contamination, we used GFP-Myh7 mice, in which slow-type muscle fibers express green fluorescent protein (GFP), allowing easy discrimination between GFP-positive slow-type fibers and GFP-negative fast-type fibers under fluorescence microscopy. Here, we profiled miRNA expression in two muscle fiber types in GFP-Myh7 mice. Microarray analysis showed that 18 and 12 miRNAs were highly expressed in slow-type and fast-type fibers, respectively, with >2 log2 fold-change (log2FC) relative to their counterparts. These distinct miRNA expressions were largely consistent with polymerase chain reaction (PCR) results. Gene ontology analyses predicted that target genes of these miRNAs were mainly involved in "regulation of transcription" in slow-type muscle fibers, and in "extracellular matrix (ECM)"-related terms in fast-type fibers. Our results suggest that distinct miRNA expression patterns in each fiber type may participate in modulating fiber-type-specific intracellular and extracellular environments.NEW & NOTEWORTHY Skeletal muscle comprises fast and slow fiber types, which reflect physiological and metabolic features. Although identifying fiber types without PCR or antibody-based assays was challenging, we visually isolated slow- and fast-type fibers from GFP-Myh7 mice, in which slow-type fibers express green fluorescent protein (GFP). Using these mice, we successfully profiled miRNA expression in precisely distinguished slow- and fast-type fibers to capture fiber-type-dependent miRNA expression. DOI： 10.1152/physiolgenomics.00023.2025 PubMed
• Misaki Yoshikuni, Kazuki Iguchi, Yasuhiro Otoguro, Shun Sato, Hirotaka Nagai, Yasuo Takemura, Arata Banno, Takuma Higuchi, Shuji Sakamoto, Susumu Muroya, Kazutsugu Matsukawa . Chiral de2025 · 掲載種別： 研究論文（学術雑誌） DOI： 10.1016/j.jfca.2025.107722
• Two-dimensional gel analysis of troponin T isoforms and fragments during postmortem aging2023 · 52nd International Congress of Meat Science and Technology: Harnessing and exploiting global opportunities 2023年8月 （ ISBN: 9789086865796, 9789086860104 ）
• Establishment of subcutaneous preadipocyte clonal line from Chinese Meishan pig2023 · 52nd International Congress of Meat Science and Technology: Harnessing and exploiting global opportunities 2023年8月 （ ISBN: 9789086865796, 9789086860104 ）

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• 畜産科学基礎特論（鹿児島大学）2025 · 2025年4月 - 現在

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