Kagoshima University · Cooperative Division, Integrated Science Domain · 教授

Research keywords寄生虫学・島嶼・衛生動物学・ブユ・蚊・Onchocerca・Simulium

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• 奄美の生物多様性と人々の暮らし2026 · 南方新社 2026年3月 （ ISBN: 978-4-86124-559-6 ）
• Metabolomic analysis of larval stages of Onchocerca japonica (Spirurida: Onchocercidae), raised in black fly (Diptera: Simuliidae) vectors, by gas chromatography-tandem mass spectrometry.2025
• Fukuda M., Otsuka Y., Sakai K., Uni S., Junker K., Saeung A., Srisuka W., Takaoka H. . Metabolomic analysis of larval stages of Onchocerca japonica (Spirurida: Onchocercidae), raised in black2025 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Acta Tropica To monitor and prevent the spread of zoonotic onchocerciasis, identification of the natural vectors (blood-sucking insects) of its causative agents, Onchocerca species, is crucial. To date, vector identification depends on the detection of infective third-stage larvae in insects by traditional dissection. We aimed to develop a novel, more efficient method for the discrimination of the four larval stages, i.e. microfilariae (Mf), first-stage larvae (L1), second-stage larvae (L2), and third-stage larvae (L3), of O. japonica by metabolomic analysis. Microfilariae of O. japonica, the causative agent of zoonotic onchocerciasis in Japan, were obtained from skin snips of wild boars and injected into newly-emerged black flies (Diptera: Simuliidae) to enable further larval development. Metabolites obtained from Mf, L1, L2, and L3 were analyzed using a gas chromatography-tandem mass spectrometer. Multivariate analysis of the data of metabolites showed the complete separation of the four larval stages. The highest level of acetoacetic acid and hydroxylamine was found in Mf and L3, respectively. Consequently, we propose that hydroxylamine is a potential marker to detect infective larvae of O. japonica in natural infections and could be a valuable tool in vector surveys, transmission studies and epidemiological surveys. DOI： 10.1016/j.actatropica.2025.107541 DOI： 10.1016/j.actatropica.2025.107541 Scopus PubMed
• Adler P.H., Low V.L., Tan T.K., Takaoka H., Otsuka Y. . Genetic relationships of three species of black flies (Diptera: Simuliidae) in Taiwan . Acta Tropica259 10732024 · 担当区分： 最終著者 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Acta Tropica As an island about 150 km from the mainland, Taiwan would be expected to have endemic species. About 64 % of its 36 species of black flies are considered endemic, more than twice the level of endemicity that has been recorded for all insects on the island. To begin assessing the validity of the high level of endemism for the Simuliidae, we used giant chromosome banding patterns and cytochrome c oxidase I (COI) sequences, against a well-defined morphological backdrop, to evaluate three of Taiwan's black flies, Simulium chungi Takaoka & Huang, S. pingtungense Huang & Takaoka, and S. sakishimaense Takaoka. Molecular data revealed high similarity of populations of S. sakishimaense in Taiwan and at the type locality on Ishigaki Island, Japan, about 180 km to the east. Thus, populations referred to as S. sakishimaense in Taiwan are conspecific with typical S. sakishimaense in Japan, confirming their non-endemicity in Taiwan. Simulium sakishimaense might have reached Ishigaki by island hopping via Taiwan from the Chinese mainland. Chromosomes and the COI gene agree with morphology that S. sakishimaense is a member of the S. multistriatum species group although the chromosomal banding patterns do not indicate that it is distinct from S. fenestratum Edwards on the mainland. Although molecular sequences indicate S. sakishimaense is monophyletic, this taxon falls within the same Operational Taxonomic Unit as nine other members of the S. multistriatum group, including S. fenestratum. Simulium pingtungense, in agreement with morphology, is molecularly distinct from the 10 other analyzed members of the S. striatum species group, tentatively suggesting that it is endemic to Taiwan, pending analysis of samples from mainland China. Simulium chungi in Taiwan is chromosomally and molecularly unique, with larvae resembling those of S. saskishimaense. It is not, however, a member of either the S. multistriatum or S. striatum species groups. For now, the S. chungi species group remains a legitimate taxon consisting of two species. Strengthening the case for endemic taxa in Taiwan awaits analysis of key samples from the Chinese mainland. DOI： 10.1016/j.actatropica.2024.107399 Scopus PubMed

• ミクロネシアにおける地域住民主導による蚊媒介性感染症対策の確立研究代表者研究2022 · 担当区分： 研究代表者 資金種別： 競争的資金
• 北米に侵入したデング熱媒介蚊であるヒトスジシマカの日本由来説の集団遺伝学的検証
• オセアニアにおける住民参加型による持続可能なデング熱対策の実践
• ミクロネシアにおける地域住民主導による蚊媒介性感染症対策の確立

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