Kagoshima University · Graduate School of Medical and Dental Sciences · 特任助教

Research keywordsミッドカイン・外傷性脳損傷・老化

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• Tani A., Sakakima H., Otsuka S., Mizuno K., Nakanishi K., Norimatsu K., Takada S., Matsuoka T., Matsuzaki R., Nakakogawa T., Maruyama I. . Stimulation of functional recovery via neurorepair me2022 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Journal of Ethnopharmacology Ethnopharmacological relevance: Ninjin'yoeito (NYT), a traditional Japanese Kampo medicine consisting of 12 herbs, has been reported to improve cognitive dysfunction, depression, and neurological recovery in patients with neurovascular diseases such as Alzheimer's disease and stroke. Several studies have reported that the NYT components exert neurotrophic, neurogenic, and neuroprotective effects. In addition, exercise enhances neuroprotection and functional recovery after stroke. Rehabilitative exercises and pharmacological agents induce neurophysiological plasticity, leading to functional recovery in stroke patients. These reports indicate that NYT treatment and exercise may promote functional recovery following stroke through their beneficial effects. However, no study has determined the effects of NYT and the possible mechanisms of neurorepair and functional recovery after stroke. Aim of the study: This study aimed to investigate the combined effects of NYT and exercise on neuroprotection and functional recovery and the underlying mechanisms in a rat ischemic stroke model. Materials and methods: Stroke was induced with 60-min middle cerebral artery occlusion (MCAO) followed by reperfusion in adult male Sprague-Dawley rats. After stroke, the rats were assigned to four groups: ischemia reperfusion (IR), NYT, exercise (Ex), and NYT + Ex. NYT-treated rats were fed a diet containing 1% NYT one day after stroke. Exercise was performed using a motorized treadmill for 5 days a week (8–15 m/min, 20 min/day), starting 3 days after stroke. The NYT treatment and exercise were continued for 4 weeks after the stroke. Infarct volume, neurological deficits, sensorimotor functions, expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), tropomyosin receptor kinase A (TrkA) and B (TrkB), caspase-3 activity, and the p-Akt/Akt ratio were examined by immunohistochemistry and western blotting. Results: Compared to the IR group, all treated groups indicated reduced infarct volumes. The NYT + Ex group showed significantly improved waking time and beam walking score compared with the IR group. The expression of NGF/TrkA/p-TrkA and BDNF/TrkB was significantly increased in the NYT + Ex group compared with those in the IR group, whereas the number of caspase-3 positive cells around the lesion was significantly lower in the NYT + Ex group than in the IR group. In addition, the ratio of p-Akt/Akt was significantly higher in the NYT + Ex group than in the IR group. Conclusions: This study suggests that NYT in combination with exercise provides neuroprotective effects and improves sensorimotor function by stimulating NGF/TrkA and BDNF/TrkB, and by activating the Akt pathway in ischemic stroke of rats. NYT may be an effective adjunctive agent in post-stroke rehabilitation. DOI： 10.1016/j.jep.2022.115927 Scopus PubMed
• 則松 貢輔, 中西 和毅, 谷 明, 松岡 輝樹, 松崎 凌真, 中小川 智美, 大塚 章太郎, 高田 聖也, 榊間 春利2021 · 記述言語： 日本語 出版者・発行元： コ・メディカル形態機能学会
• Mito T., Onodera Y., Otsuji M., Hirano N., Takahata K., Yanagi N., Iwamoto A., Hamaguchi S., Takada S., Baba T., Chikumoto N., Kawagoe A., Kawanami R. . Improvement of Icdegradation2021 · 記述言語： 日本語 出版者・発行元： IEEE Transactions on Applied Superconductivity As a high-temperature superconducting (HTS) conductor with a large current capacity applicable to a nuclear fusion experimental device, REBCO (REBa2CuOy) tapes and high-purity aluminum sheets are alternately laminated, placed in a groove of an aluminum alloy jacket having a circular cross section, and the lid is friction-stir welded. To make the current distribution and mechanical characteristics uniform, the conductor is twisted at the end of the manufacturing process. In the early prototype conductor, when the Ic was measured in liquid nitrogen under self-magnetic field conditions, Ic degradations were observed from the beginning, and the characteristic difference between the two prototype samples under the same manufacturing conditions were large. Furthermore, Ic degradation was progressed by repeating the thermal cycle from room temperature to liquid nitrogen temperature. This Ic degradation did not occur uniformly in the longitudinal direction of the conductor but was caused by local Ic degradation occurring at multiple locations. If the conductor was not manufactured uniformly in the longitudinal direction, the difference in thermal shrinkage between the REBCO tape and the aluminum alloy jacket caused local stress concentration in the REBCO tape and buckling occurred. Element experiments to explain this mechanism were conducted to clarify the conditions under which Ic degradation due to buckling occurs. Then prototype conductors were tested with improved manufacturing methods, and as a result, Ic degradation could be suppressed to 20% or less. We have achieved the prospect of producing a conductor with uniform characteristics in the longitudinal direction. DOI： 10.1109/TASC.2021.3069923 Scopus
• Nakanishi K. . Effect of low-intensity motor balance and coordination exercise on cognitive functions, hippocampal Aβ deposition, neuronal loss, neuroinflammation, and oxidative stress in a mo2020 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Experimental Neurology It is well known that physical exercise reduces the risk of Alzheimer's disease (AD) and age-related cognitive decline. However, its mechanisms are still not fully understood. This study aimed to investigate the effect of aging and rotarod exercise (Ex) on cognitive function and AD pathogenesis in the hippocampus using senescence-accelerated mice prone 8 (SAMP8). Cognitive functions clearly declined at 9-months of age. Amyloid-beta (Aβ) deposition, neuronal loss, and glia activation-induced neuroinflammation increased with aging. The rotarod Ex prevented the decline of cognitive functions corresponding to the suppression of Aβ deposition, neuroinflammation, neuronal loss, inducible nitric oxide synthase (NOS) activities, and neuronal NOS activities. In addition, the rotarod Ex suppressed proinflammatory M1 phenotype microglia and A1 phenotype astrocytes. Our findings suggest that low-intensity motor balance and coordination exercise prevented age-related cognitive decline in the early stage of AD progression, possibly through the suppression of hippocampal Aβ deposition, neuronal loss, oxidative stress, and neuroinflammation, including reduced M1 and A1 phenotypes microglia and astrocytes. DOI： 10.1016/j.expneurol.2020.113590 Scopus PubMed

• ミッドカイン発現調節と運動療法の併用による外傷性脳損傷の新規治療に関する研究2022 · 配分額： 4550000円 （ 直接経費： 3500000円 、 間接経費： 1050000円 ）
• 栄養因子ミッドカインの発現調節と運動療法の併用による新規の外傷性脳損傷治療の研究2020 · 2020年10月 - 現在
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