Kagoshima University · Division of Veterinary Medicine, School of Agriculture, Fisheries and Veterinary Medicine · 准教授

Research keywordsparamyxovirus biology・parainfluenza virus type 2・viral polymerase・nucleoprotein mutations・nanopore sequencing・viral transcription regulation

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• Yusuke Masuda, Tofazzal Md Rakib, Lipi Akter, Keisuke Nakagawa, Kiyotada Naitou, Akatsuki Saito, Ryoji Yamaguchi, Yusuke Matsumoto . Nanopore sequencing-based measurement of paramyxovir2025 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） Paramyxovirus polymerase recognizes an RNA editing signal on the viral genome and transcribes mRNA in which guanine nucleotides are inserted in a template-independent manner. This enables the synthesis of multiple proteins from a single gene, which is important for viral growth. We developed a method to quantify RNA editing efficiency using Oxford Nanopore Technologies' MinION platform. We performed sequence analysis of reverse transcription-PCR amplicons with the RNA editing sites in cells infected with Sendai virus (SeV) and canine distemper virus (CDV). By modifying reverse transcription primers, we simultaneously assessed RNA editing efficiency in mRNA, antigenome and genome. We observed distinct differences in mRNA editing efficiency between SeV and CDV. Notably, while RNA editing in SeV is confined to mRNA, in CDV it is also observed in antigenome/genome. (Anti)genomes harboring extra nucleotides may deviate from a multiple-of-six sequence, suggesting that RNAs not following the "Rule of Six" are produced in CDV-infected cells. DOI： 10.1016/j.virol.2025.110572 PubMed
• Yusuke Matsumoto, Tomoko Honda, Fumihiko Yasui, Akinori Endo, Takahiro Sanada, Sakiko Toyama, Asako Takagi, Tsubasa Munakata, Risa Kono, Kenzaburo Yamaji, Naoki Yamamoto, Yasushi Saeki, Michinori Koha2024 · 担当区分： 筆頭著者 記述言語： 英語 掲載種別： 研究論文（学術雑誌） INTRODUCTION: To analyze the molecular pathogenesis of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a small animal model such as mice is needed: human angiotensin converting enzyme 2 (hACE2), the receptor of SARS-CoV-2, needs to be expressed in the respiratory tract of mice. METHODS: We conferred SARS-CoV-2 susceptibility in mice by using an adenoviral vector expressing hACE2 driven by an elongation factor 1α (EF1α) promoter with a leftward orientation. RESULTS: In this model, severe pneumonia like human COVID-19 was observed in SARS-CoV-2-infected mice, which was confirmed by dramatic infiltration of inflammatory cells in the lung with efficient viral replication. An early circulating strain of SARS-CoV-2 caused the most severe weight loss when compared to SARS-CoV-2 variants such as Alpha, Beta and Gamma, although histopathological findings, viral replication, and cytokine expression characteristics were comparable. DISCUSSION: We found that a distinct proteome of an early circulating strain infected lung characterized by elevated complement activation and blood coagulation, which were mild in other variants, can contribute to disease severity. Unraveling the specificity of early circulating SARS-CoV-2 strains is important in elucidating the origin of the pandemic. DOI： 10.3389/fimmu.2024.1440314 PubMed
• Shoko Kanno, Yusuke Matsumoto2023 · 担当区分： 責任著者 記述言語： 英語
• Lipi Akter, Ryo Matsumura, Daisuke Kobayashi, Hiromichi Matsugo, Haruhiko Isawa, Yusuke Matsumoto . Segment-specific promoter activity for RNA synthesis in the genome of Oz virus, genus2023 · 担当区分： 責任著者 記述言語： 英語 掲載種別： 研究論文（学術雑誌） Oz virus (OZV), a tick-borne, six-segmented negative-strand RNA virus in the genus Thogotovirus, caused a fatal human infection in Japan in 2023. To study viral RNA synthesis, we developed an OZV minigenome assay using mammalian cells. This revealed variations in promoter activities among the six genome segments. The "distal duplex," a double-stranded RNA structure beginning at the 11th nucleotide on the 5' end and the 10th on the 3' end, was found in all segments. A factor affecting promoter activity was the base pairing between the 12th nucleotide at the 5' end and the 11th at the 3' end, forming either G:C or A:U pairs. Disruption of this pairing caused a significant loss of promoter activity, emphasizing the importance of the distal duplex with at least six consecutive base pairs. Comparative analysis of genome terminal sequences suggests similar structural variations in the promoters of other species in Thogotovirus. DOI： 10.1016/j.virol.2025.110410 PubMed

• 人工的遺伝子操作法による革新的おたふくかぜワクチンの開発2024 · 担当区分： 研究代表者
• Rule of Sixに従わないウシパラミクソウイルスを用いたワクチン開発研究2024 · 担当区分： 研究代表者 配分額： 4680000円 （ 直接経費： 3600000円 、 間接経費： 1080000円 ）
• ウイルス増殖レベルの人工操作による新規牛パラミクソウイルス感染症ワクチン開発2024 · 担当区分： 研究代表者
• パラミクソウイルスゲノム複製におけるRule of Six存在意義の証明2024 · 担当区分： 研究代表者

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