Kagoshima University · Faculty of Fisheries · 教授

Research keywordsFish and Crustacean nutrition・魚類・甲殻類栄養・魚類栄養・甲殻類栄養・飼料添加物・成長性能・免疫応答・ストレス耐性

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• Mzengereza K., Ishikawa M., Koshio S., Shadrack R.S., Zhang Y., Dossou S., Kotani T., Shahin S.A., Zaineldin A.I., Waqalevu V., Dawood M.A.O., Hassan A.M., Al-Sharif M.M., El Basuini M.F. . Re2022 · 記述言語： 日本語 出版者・発行元： Aquaculture Reports The current work assessed the inclusion impacts of oilseed camelina meal (CM) as a protein source in red sea bream (Pagrus major) diets. A 45 day assessment period with 180 juveniles (6.47 ± 0.17 g) were allocated in triplicates to 4 experimental groups and fed formulated diets in which fish meal (FM) was subrogated at graded series of 0% (T1), 20.5% soybean meal (T2), 20.5% camelina meal (T3), and 33% camelina meal (T4). No noticeable alterations were observed in specific growth rate, feed intake, survival rate, hepatosomatic index, and Fulton's condition factor among the experimental groups. Fish fed the T4 diet showed considerably reduced (P < 0.05) final weight and body protein content when compared to those fed T2, T3, and basal diets. Plasma biochemical parameters show no differences (P > 0.05) in glucose, total bilirubin, total protein, total cholesterol, triglyceride, glutamyl oxaloacetic transaminase, and glutamic pyruvate transaminase. Hematocrit levels decreased noticeably (P < 0.05) in fish groups fed camelina meal in comparison to the control group. Liver and intestinal histology showed a healthy status and an increase in villus length and goblet cell number in camelina groups. Fish fed the T4 diet displayed higher expression levels (P < 0.05) of relative mRNA interleukin 1 beta (IL-1b) and tumor necrosis factor-alpha (TNF-α) in comparison to other groups. The inclusion of camelina meal (up to 20%) in red sea bream diets produced similar outcomes to specimens fed fish meal and soybean meal. DOI： 10.1016/j.aqrep.2022.101175 Scopus
• Matsui H. . DHA Accumulation in the Polar Lipids of the Euryhaline Copepod Pseudodiaptomus inopinus and Its Transfer to Red Sea Bream Pagrus major Larvae . Frontiers in Marine Science82021 · 記述言語： 日本語 出版者・発行元： Frontiers in Marine Science The euryhaline copepod Pseudodiaptomus inopinus play important roles in coastal waters as vectors of docosahexanoic (DHA) and eicosapentaenoic (EPA) acids for larval fish. While DHA and EPA in polar lipids (PLs) are more effective for fish larval development than non-polar lipid forms (NLs), there is little knowledge how much these lipids are accumulated in copepods from microalgae and are effective for early development of fish larvae. We report PLs fatty acid profiles of P. inopinus fed DHA-poor microalgae and evaluate its significance as a food source for larvae development of Pagrus major, compared with DHA-enriched rotifers. Copepods and rotifers were fed a mixed diet of three algal species (Phaeodactylum tricornutum, Tisochrysis lutea, and Pavlova lutheri), in addition of DHA-supplemented Super Fresh Chlorella (SFC) for rotifers. Compared with SFC, the algal mixture had higher EPA but lower DHA. Copepods had higher DHA and EPA in total lipids than rotifers fed each diet. Copepod PLs were specifically enriched with DHA and their contents were higher than both rotifers. On the other hand, PLs EPA contents were comparable between preys, indicating that copepods selectively fortified the PLs. Fish culture experiment showed that larvae fed copepods had higher growth than those fed SFC-enriched rotifers. Principal component analysis for each organism fatty acid composition emphasized trophic modification of DHA by copepods toward larval fish. This study highlighted that P. inopinus contribute to enhanced growth of coastal larval fish by efficiently transferring DHA via copepod fatty acid metabolism. DOI： 10.3389/fmars.2021.632876 Scopus
• Khoa T.N.D. . Changes in early digestive tract morphology, enzyme expression and activity of Kawakawa tuna (Euthynnus affinis) . Aquaculture530 2021年1月 詳細を見る 記述言語： 日本語 出版2021 · 記述言語： 日本語 出版者・発行元： Aquaculture Kawakawa tuna (Euthynnus affinis) is a novel target species for aquaculture in Japan and currently there is very limited knowledge on its early ontogenetic development in captivity. The ontogeny of E. affinis larvae from hatching to 20 days after hatching (DAH) was illustrated in the present study through an integrated approach of histology, biochemistry and molecular approaches. Larvae were fed enriched L-type rotifer Brachionus plicatilis species complex from 2 to 10 DAH, followed by enriched Artemia nauplii. Target digestive enzymes; trypsin, pepsin, lipase and amylase were assessed in order to evaluate nutritional condition. The larvae began exogenous feeding from 2 DAH, coinciding with the opening of the oral capacity and anus. The yolk sac was rapidly consumed and completely resorbed at 3 DAH, while the oil globule disappeared by 5 DAH. Enzymatic activity and expression of the target enzymes were detectable at hatching, except pepsin. With the development of the gastric glands, pepsin activity was then detected at 10 DAH, which was followed by rapid increase in pepsin activity at 15 DAH. The larvae showed rapid growth, however, high mortality also occurred at 6–10 DAH which has been highlighted as a critical stage in the larviculture of E. affinis. From 15 to 20 DAH, the histological structures of the digestive tract were completely segmented with the digestive tract resembling that of its juvenile stages. The activity of trypsin, pepsin, and lipase tended to increase sharply, while amylase dramatically dropped, possibly indicating a change in dietary preference. Findings from the present study provides a preliminary insight into the early development of the digestive system and associated digestive enzymes of E. affinis larvae and establishes an initial step towards the rearing and feeding protocol optimization of this novel target species. DOI： 10.1016/j.aquaculture.2020.735935 Scopus
• Hossain M.S. . Long term feeding effects of functional supplement ‘cytidine monophosphate’ on red sea bream, Pagrus major performances . Aquaculture533 2021年2月 詳細を見る 記述言語2021 · 記述言語： 日本語 出版者・発行元： Aquaculture There is no definitive evidence associated with administration duration of nucleotide on aquaculture organisms. This study evaluates the long-term feeding effects of cytidine monophosphate (CMP) nucleotide as functional supplement on growth and health conditions of red sea bream, Pagrus major. A formulated basal diet added with 0% (control), 0.2% (CMP-0.2) and 0.4% (CMP-0.4) individual CMP nucleotide to prepare three experimental feeds. Each experimental feed was fed to triplicate tanks of red sea bream (3.0 ± 0.01 g) for 150 days. At day 50th, growth performances were influenced significantly by dietary CMP inclusion and fish fed CMP-0.4 diet showed significantly higher value. In day 100 and 150 growth performances also non-significantly enhanced by CMP supplementation. Feed utilization performances also showed improved values in nucleotide included diets compared to the non-supplemented control diet (P > 0.05). No significant changes observed on measured hematological parameters by nucleotide administration duration. Lysozyme activity (LA) and total serum protein (TSP) significantly rose to their highest level on 50 days in CMP-0.4 diet group. In 100 and 150th day supplementation also improved TSP and LA values and it reaches maximum in CMP-0.2 diet group (P > 0.05). Oxidative radical production (nitro-blue-tetrazolium assay) and the activity of peroxidase and catalase were not affected significantly with feeding nucleotide in all sampling days. Supplementation of nucleotide improved oxidative stress tolerance capacity. Remarkably, CMP-0.2 diet group showed the least oxidative stress condition in 50th and 100th day. In 150th day fish showed acceptable but reduced oxidative stress resistances. On the basis of the overall findings, finally we concluded that, CMP nucleotide administration regime influences the optimum growth, health performance and immune response of Pagrus major and its mostly after feeding for 50 days, but not after 100 days. DOI： 10.1016/j.aquaculture.2020.736150 Scopus

• 魚類および甲殻類における栄養素の消化吸収と代謝1996 · 資金種別： 競争的資金
• Digestibility and metabolism of nutrients in marine animal資金種別： 競争的資金

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