Kagoshima University · Faculty of Agriculture · 教授

Research keywordsOrnamental Horticulture and Floriculture・茶ポリフェノール・花き園芸・天然有機化合物・化学分類・Tea polyphenol・Plant Chemosystematics・Natural products・フラボノイド・植物化学分類学

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• Huynh T.P., Motoyama C., Oshima H., Kozai N., Hashimoto F., Shimizu K. . Functional analysis of flavonoid 3’,5’-hydroxylase gene using transgenic Japanese morning glory (Ipomoea nil) .2024 · 記述言語： 日本語 出版者・発行元： Acta Horticulturae Delphinidin (Dp), an anthocyanin, is a blue and purple pigment present in many flowers of high economic value in ornamental horticulture. However, some ornamental species, such as chrysanthemums, lilies, roses, and carnations, lack the flavonoid 3’,5’-hydroxylase (F3’5’H) gene involved in delphinidin biosynthesis and, therefore, lack Dp and do not have blue-flowered cultivars. Recently, F3’5’H genes have been isolated from plants with blue and purple flowers that accumulate Dp and the transgenic plants expressing this gene have been produced. However, compatibility exists between the exogenous F3’5’H genes and the host plant, making the Dp accumulation trait plant specific. For example, the introduction of Campanula medium F3’5’H into chrysanthemums produced plants with high Dp accumulation, whereas F3’5’H from other plants, such as Gentiana triflora, resulted in reduced Dp accumulation. Several studies on roses have also reported similar compatibility. In this study, we used Ipomoea nil, which lacks the F3’5’H gene, to clarify the mechanisms underlying the compatibility between the F3’5’H gene and the host plant. Compared with chrysanthemums and roses, I. nil has advantages such as high efficiency in genetic transformation and a short interval between the transformation and flowering of regenerated plants. The accumulation of Dp-type anthocyanins was high when F3’5’H genes isolated from Eustoma grandiflorum, C. medium, and × Viola wittrockiana were introduced into I. nil but low when the gene was from G. triflora. Unexpectedly, the petals of Dp-accumulated transgenic plants did not develop adequately, and the buds did not open. Observations of petal cells revealed that enlargement, necessary for flowering, was inhibited. In conclusion, this study revealed that the transgenic I. nil is valuable for the functional analysis of the F3’5’H gene. DOI： 10.17660/ActaHortic.2024.1404.117 Scopus
• 橋本 文雄 . 花と健康 . 口腔衛生学会雑誌73 ( 2 ) 90 - 96 2023年 詳細を見る 記述言語： 日本語 出版者・発行元： 一般社団法人 口腔衛生学会 DOI： 10.5834/jdh.73.2_902023 · 記述言語： 日本語 出版者・発行元： 一般社団法人 口腔衛生学会 DOI： 10.5834/jdh.73.2_90 CiNii Research
• Wang Zitai, Okutsu Kayu, Yoshizaki Yumiko, Futagami Taiki, Tamaki Hisanori, Maruyama Takuro, Toume Kazufumi, Komatsu Katsuko, Hashimoto Fumio, Takamine Kazunori2022 · 記述言語： 英語 出版者・発行元： John Wiley & Sons Australia, Ltd
• Zitai Wang, Kayu Okutsu*, Taiki Futagami, Yumiko Yoshizaki, Hisanori Tamaki, Takuro Maruyama, Kazufumi Toume, Katsuko Komatsu, Fumio Hashimoto and Kazunori Takamine.2020 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌）

• トルコギキョウの花色育種に関する研究2014 · トルコギキョウの花色育種に関する研究
• チューリップの化学分類に関する研究2011 · チューリップの化学分類に関する研究
• B環配糖化デルフィニジンとフラボノールとの相互作用によるトルコギキョウの花色改変
• 茶ポリフェノールに関する研究

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