Kagoshima University · Graduate School of Medical and Dental Sciences · 助教

Research keywordsGap repair クローニング・酵母相同組換え・パターン認識受容体・免疫

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• Iizasa E., Iwai H., Oyamada Y., Hamashima K., Nishi R., Ataka K., Amitani H., Ohinata K., Kato I., Asakawa A. . A plant-derived δ opioid receptor agonist rubiscolin-6 ameliorates sickness beha2024 · 担当区分： 筆頭著者 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Journal of Functional Foods Cancer cachexia, which encompasses a range of sickness behaviors such as anorexia, reduced locomotor activity, and depression, diminishes quality of life and increases mortality in cancer patients. Rubiscolin-6 is a hexapeptide derived from ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), a major plant leaf protein. Previous reports have demonstrated that rubiscolin-6 functions as an agonist of the δ opioid receptor, inducing diverse effects such as orexigenic, antinociceptive, and antidepressant-like effects. In this study, we investigated the effects of rubiscolin-6 on sickness behavior in colon26-bearing cachexia model mice. Intraperitoneal administration of rubiscolin-6 enhanced food intake in cachexic mice with chronic anorexia. It also increased locomotor activity, which decreased in cachexic mice, as assessed by the open field test. Moreover, the tail suspension test revealed that rubiscolin-6 alleviated depression-like behavior in mice with cachexia. These findings imply that rubiscolin-6 could be used to ameliorate the sickness behavior associated with cancer cachexia. DOI： 10.1016/j.jff.2024.106297 Scopus
• Mitsuyama H., Iizasa E., Kukita A., Toda S., Yoshida H., Inoue H., Hara H. . Deletion of Card9 eliminates the detrimental facets of mycobacterial adjuvants . Heliyon10 (2024 · 記述言語： 日本語 掲載種別： 研究論文（学術雑誌） 出版者・発行元： Heliyon Although mycobacterial adjuvants are capable of eliciting a strong adaptive humoral and cellular immunity, they also sometimes provoke detrimental outcomes, including autoimmune/inflammatory syndromes. Here, we show that the deletion of caspase recruitment domain family member 9 (Card9), a signaling adaptor of a set of innate immune receptors, can eliminate the detrimental effects of mycobacterial adjuvants. Long-lasting tissue-destructive skin inflammation at the site of complete Freund's adjuvant (CFA) injection, lung granuloma formation induced by intratracheal Mycobacterium bovis Bacillus Calmette-Guérin infection, and the incidence and severity of experimental autoimmune encephalomyelitis and collagen-induced arthritis induced by autoantigen immunization with CFA were considerably attenuated in Card9-deficient (Card9−/−) mice compared to control wild-type mice. Card9−/− mice showed impaired development of Th17, but not Th1, in the early phase after autoimmune induction, due to the impaired development of IL-6-producing Sirpαhigh dendritic cells, which are essential for priming pathigenic Th17, in the draining lymph nodes. However, Card9 deletion did not affect overall adaptive antibody production or delayed-type hypersensitivity following immunization with CFA, indicating that humoral and type 1 immune responses remained intact. These results suggest that avoiding the activation of Card9 signaling during vaccination with mycobacteria-containing vaccines may mitigate the risk of detrimental type 3 immune responses, while preserving type 1 immune responses that are effective against intracellular pathogens and cancers. DOI： 10.1016/j.heliyon.2024.e38139 Scopus PubMed
• Kubota M, Iizasa E, Chuuma Y, Kiyohara H, Hara H, Yoshida H . Adjuvant activity of Mycobacteria-derived mycolic acids. . Heliyon6 ( 5 )2020 · DOI： 10.1016/j.heliyon.2020.e04064 PubMed
• Phongsisay V., Iizasa E., Hara H., and Yoshida Y.2017 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌）

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