Kobe University · Graduate School of Health Sciences

Research keywordsantiviral screening・hepatitis C virus・hepatitis B virus・dengue virus・medicinal plants・SARS-CoV-2 replicons・gallic acid derivatives・viral lifecycle assays

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• Antiviral activity of Indonesian Medicinal Plants against hepatitis B virus.2020 · 2020年, Pharmacognosy Journal, 12(5) (5), 1108 - 1114
• Smp76, a Scorpine-Like Peptide Isolated from the Venom of the Scorpion Scorpio maurus palmatus, with a Potent Antiviral Activity Against Hepatitis C Virus and Dengue Virus2019 · Springer Science and Business Media LLC, 2019年01月, International Journal of Peptide Research and Therapeutics, 26(2) (2), 811 - 821
• Construction of Fosmid-based SARS-CoV-2 replicons for antiviral drug screening and replication analyses in biosafety level 2 facilities.2019 · The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has necessitated the global development of countermeasures since its outbreak. However, current therapeutics and vaccines to stop the pandemic are insufficient and this is mainly because of the emergence of resistant variants, which requires the urgent development of new countermeasures, such as antiviral drugs. Replicons, self-replicating RNAs that do not produce virions, are a promising system for this purpose because they safely recreate viral replication, enabling antiviral screening in biosafety level (BSL)-2 facilities. We herein constructed three pCC2Fos-based RNA replicons lacking some open reading frames (ORF) of SARS-CoV-2: the Δorf2-8, Δorf2.4, and Δorf2 replicons, and validated their replication in Huh-7 cells. The functionalities of the Δorf2-8 and Δorf2.4 replicons for antiviral drug screening were also confirmed. We conducted puromycin selection following the construction of the Δorf2.4-puro replicon by inserting a puromycin-resistant gene into the Δorf2.4 replicon. We observed the more sustained replication of the Δorf2.4-puro replicon by puromycin pressure. The present results will contribute to the establishment of a safe and useful replicon system for analyzing SARS-CoV-2 replication mechanisms as well as the development of novel antiviral drugs in BSL-2 facilities.
• Antiviral Activities of Curcuma Genus against Hepatitis C Virus2018 · 2018年12月, NATURAL PRODUCT COMMUNICATIONS, 13(12) (12), 1579 - 1582

• B型肝炎カプシドタンパク質を標的とした新規阻害剤の創製と性状解析に関する研究 靭 千恵, 亀岡 正典 日本学術振興会, 科学研究費助成事業, 基盤研究(C), 神戸大学, 2025年04月01日 - 2028年03月31日2025 · KUID parsed section
• 新型コロナウイルス感染増殖における脂質代謝系の役割の解明 靭千恵 木下基礎科学研究基金助成事業, 2021年08月 - 2023年03月, 研究代表者2021 · KUID parsed section
• C 型肝炎ウイルス感染粒子形成を阻害するバイオプローブを用いたアポリポタンパク質産生制御機構の解明研究 靱千恵 伊藤忠兵衛基金, 2020年04月 - 2021年03月2020 · KUID parsed section
• インドネシアの薬用植物からの抗 B 型肝炎ウイルス新規リード化合物の探索と開発研究 靭 千恵 日本学術振興会, 二国間交流事業, 2018年04月 - 2021年03月, 研究代表者 競争的資金2018 · KUID parsed section

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