Kobe University · Graduate School of Science · 准教授

Research keywords植物生理学・ケミカルバイオロジー・光生物学・植物分子

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• Discovery of 2,6-Dihalopurines as Stomata Opening Inhibitors: Implication of an LRX-Mediated H+-ATPase Phosphorylation Pathway2023 · 2023年02月, ACS Chemical Biology
• Global Shifts in Gene Expression Profiles Accompanied with Environmental Changes in Cnidarian-Dinoflagellate Endosymbiosis.Stable endosymbiotic relationships between cnidarian animals and dinoflagellate algae are vital for sustaining coral reef ecosystems. Recent studies have shown that elevated seawater temperatures can cause the collapse of their endosymbiosis, known as 'bleaching', and result in mass mortality. However, the molecular interplay between temperature responses and symbiotic states still remains unclear. To identify candidate genes relevant to the symbiotic stability, we performed transcriptomic analyses under multiple conditions using the symbiotic and apo-symbiotic (symbiont free) Exaiptasia diaphana, an emerging model sea anemone. Gene expression patterns showed that large parts of differentially expressed genes in response to heat stress were specific to the symbiotic state, suggesting that the host sea anemone could react to environmental changes in a symbiotic state-dependent manner. Comparative analysis of expression profiles under multiple conditions highlighted candidate genes potentially important in the symbiotic state transition under heat-induced bleaching. Many of these genes were functionally associated with carbohydrate and protein metabolisms in lysosomes. Symbiont algal genes differentially expressed in hospite encode proteins related to heat shock response, calcium signaling, organellar protein transport, and sugar metabolism. Our data suggest that heat stress alters gene expression in both the hosts and symbionts. In particular, heat stress may affect the lysosome-mediated degradation and transportation of substrates such as carbohydrates through the symbiosome (phagosome-derived organelle harboring symbiont) membrane, which potentially might attenuate the stability of symbiosis and lead to bleaching-associated symbiotic state transition.
• Photoinduced Synthesis of Thiocyanates through Hydrogen Atom Transfer and One-Pot Derivatization to Isothiocyanates.Photoinduced benzylic C-H thiocyanation is described. A series of alkyl thiocyanates were efficiently obtained by using Selectfluor as the oxidant. Moreover, we accomplished the one-pot isothiocyanation following the C-H thiocyanation. The thiocyanates and isothiocyanates were applied to the divergent transformation of pharmaceuticals.
• Image-based quantification of Arabidopsis thaliana stomatal aperture from leaf images.The quantification of stomatal pore size has long been a fundamental approach to understand the physiological response of plants in the context of environmental adaptation. Automation of such methodologies not only alleviates human labor and bias, but also realizes new experimental research methods through massive analysis. Here, we present an image analysis pipeline that automatically quantifies stomatal aperture of Arabidopsis thaliana leaves from brightfield microscopy images containing mesophyll tissue as noisy backgrounds. By combining a YOLOX-based stomatal detection submodule and a U-Net-based pore segmentation submodule, we achieved 0.875 mAP50 (mean average precision; stomata detection performance) and 0.745 IoU (intersection of union; pore segmentation performance) against images of leaf discs taken with a brightfield microscope. Moreover, we designed a portable imaging device that allows easy acquisition of stomatal images from detached/undetached intact leaves on-site. We demonstrated that this device in combination with fine-tuned models of the pipeline we generated here provides robust measurements that can substitute for manual measurement of stomatal responses against pathogen inoculation. Utilization of our hardware and pipeline for automated stomatal aperture measurements is expected to accelerate research on stomatal biology of model dicots.

• 天然物生物学：植物二次代謝産物が有する生物学的意義の体系化 瀬戸 義哉, 佐藤 道大, 杉山 龍介, 白川 一, 相原 悠介 日本学術振興会, 科学研究費助成事業, 学術変革領域研究(B), 明治大学, 2024年04月01日 - 2027年03月31日2024 · KUID parsed section
• 一細胞レベルでの高解像度発現解析と代謝産物の標的同定による天然物生物学 白川 一, 相原 悠介 日本学術振興会, 科学研究費助成事業, 学術変革領域研究(B), 奈良先端科学技術大学院大学, 2024年04月01日 - 2027年03月31日2024 · KUID parsed section
• 植物修飾分子による多面的機能のテイラーメイド制御 科学技術振興機構(JST), 戦略的創造研究推進事業 さきがけ 植物分子の機能と制御, 名古屋大学, 2022年10月 - 2026年03月, 研究代表者2022 · KUID parsed section
• 植物の特化代謝物による新規の翻訳後修飾機構 科学技術振興機構(JST), 戦略的創造研究推進事業 ACT-X 生命と化学, 名古屋大学, 2019年10月 - 2023年03月, 研究代表者2019 · KUID parsed section

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