University of the Ryukyus · United Graduate School of Agricultural Sciences, Kagoshima University · 教授

Research keywords体外受精・家畜・受精卵移植・卵子・凍結・クローン・胚発生・繁殖工学・精子・成熟

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• Interference with the interaction between sperm arylsulfatase A and sulfated carbohydrate chain residues of the zona pellucida effectively suppresses polyspermy during in vitro fertilization of porcine oocytes2025 · Sakamura S, Kawasaki K, Yamanaka K, Tatemoto H
• Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte-cumulus cell communication2025 · Abstract Purpose In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte‐cumulus cell communication during the in vitro growth (IVG) of bovine oocytes. Methods Oocyte‐granulosa cell complexes (OGCs) were harvested from slaughterhouse‐derived ovaries and grown in vitro for 5 d at 38.5°C in 5% CO₂ humidified air. Melatonin (10⁻⁷, 10⁻⁹, or 10⁻¹¹ M) was added to the culture medium. Results Oocyte diameter increased on day 5 from its initial value in all groups. The antrum formation rate was significantly higher in the 10⁻⁹ M melatonin‐treated group than in the control. The melatonin‐treated group showed reduced oxidative stress and increased gap junction communication compared with the control. ER stress‐related genes in OGCs were significantly downregulated in the 10⁻⁹ M melatonin‐treated group compared with those in the control. No significant changes were found in subsequent maturation among groups; however, 10⁻⁹ M melatonin treatment during IVG and IVM increased the maturation rate compared with that in the control. Conclusions Melatonin reduces oxidative stress, which attenuates ER stress in OGCs during IVG of bovine oocytes and may improve IVG efficiency in assisted reproductive technology.
• Attenuation of endoplasmic reticulum stress improves in vitro growth and subsequent maturation of bovine oocyte2024 · Islam MN, Ebara F, Kawasaki K, Konno T, Tatemoto H, Yamanaka K.
• The strong anti-hyaluronidase effect of ellagic acid markedly decreasespolyspermy during in vitro fertilization, resulting in sustainment of thedevelopmental potency in porcine oocytes2024 · Kawasaki K, Hirai M, Ishiki Y, Nagahama A, Konno T, Tamanaka K, Tatemoto H

• ブタ体外受精時の精子SPAM1-ARSA複合体に着目した多精子 受精抑制技術の確立2021 · 本研究は，精子SAPM1-ARSA複合体と卵透明帯との相互作用が受精機序に関与している点に着目し，その生理学的作用を制御する手法を介してブタIVF時に高頻度に起こる多精子受精を効果的に抑制することを目的として実施する。さらに，この精子SPAM1-ARSA複合体と透明帯硫酸化糖鎖との相互作用は，全ての哺乳類の受精時にも関与しており，他の哺乳類卵のIVF時の多精子受精抑制にも繋がる重要な研究であると考えられ る。さらに，当研究室ではIVF由来胚のガラス化凍結試験も実施しており，体外で正常受精胚を多数作出できるIVF環境を整えることは希少な日本唯一の在来豚であるアグーの種の保存にとっても重要である。
• ブタ卵の体外成熟に伴う透明帯の硫酸化修飾に起因した多精子受精抑制技術の確立2016 · 基盤研究(C)
• ブタ卵の活性化に伴うMPFとMAP kinaseの不活化に関する検討
• ブタ卵子への精子侵入時におけるヒアルロニダーゼの生理作用の解明

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