University of the Ryukyus · Faculty of Agriculture · 教授

Research keywordsacid・Citrinin・酵素・エネルギー代謝・science・Biochemistry・Fermentation・Lactic

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• 生物の発熱現象について2025 · 外山 博英
• 微生物機能を活用した新たな風味を有する泡盛醸造技術の開発2023 · 塚原 正俊, 山田 修, 髙木 博史, 外山 博英
• Inhibitory Effect of Water Soluble Fraction of Monascus-Fermented Rice on Lipid Accumulation in 3T3 L1 Adipocyte2022 · Indika Pradeep Wanninaika, Hirohide Toyama, Shinjiro Thachibana, Perumpuli Arachchige Buddhika Niroshie Perumpuli, Chamari Hettiarachchi and Migelhewa Nidarsha Kaumal
• Characterization of 3 phylogenetically distinct membrane-bound d-gluconate dehydrogenases of Gluconobacter spp. and their biotechnological application for efficient 2-keto-d-gluconate production.2022 · We identified a novel flavoprotein-cytochrome c complex d-gluconate dehydrogenase (GADH) encoded by gndXYZ of Gluconobacter oxydans NBRC 3293, which is phylogenetically distinct from previously reported GADHs encoded by gndFGH and gndSLC of Gluconobacter spp. To analyze the biochemical properties of respective GADHs, Gluconobacter japonicus NBRC 3271 mutant strain lacking membranous d-gluconate dehydrogenase activity was constructed. All GADHs (GndFGH, GndSLC, and GndXYZ) were successfully overexpressed in the constructed strain. The optimal pH and KM value at that pH of GndFGH, GndSLC, and GndXYZ were 5, 6, and 4, and 8.82 ± 1.15, 22.9 ± 5.0, and 11.3 ± 1.5 m m, respectively. When the mutants overexpressing respective GADHs were cultured in d-glucose-containing medium, all of them produced 2-keto-d-gluconate, revealing that GndXYZ converts d-gluconate to 2-keto-d-gluconate as well as other GADHs. Among the recombinants, the gndXYZ-overexpressing strain accumulated the highest level of 2-keto-d-gluconate, suggesting its potential for 2-keto-d-gluconate production.

• グルコノバクター属酢酸菌のソルビトール代謝工学2007 · 基盤研究(C)
• 耐熱性酢酸菌に関する研究2006 · ■■■
• no_title2006 · ■■■
• 酢酸菌の酵素と代謝に関する研究

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