Shinshu University · Faculty of Engineering · 助教

Research keywordsPleurotus ostreatus・CRISPR Cas9・genome editing・biolistic transformation・fruiting body development・marker-free editing・Rim101・cell wall formation

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• CRISPR/Cas9-directed disruption of wc-2 leads to the absence of fruiting body development in Pleurotus ostreatus.
• Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection
• Role of the putative transcription factor Rim101 in pH/oxidative stress response and cell wall structure formation in the white-rot fungus Pleurotus ostreatus
• Establishment of a CRISPR/Cas9 protocol by biolistic transformation of the filamentous basidiomycete Pleurotus ostreatus.

• 高精度かつ安心安全なキノコ育種を実現する塩基編集基盤の構築と実用品種への展開（辞退）
• 食用担子菌の遺伝子組換えに該当しない分子育種を切り拓く迅速ゲノム編集技術の開発

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