Institute of Science Tokyo · Research Institute for Future Society Creation

Research keywordscell-free gene expression・droplet microfluidics・genetic code expansion・translation elongation・D-amino acid incorporation・RNA polymerase evolution・nucleocapsid assembly

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• Comprehensive elucidation of effect of nascent peptide sequences and EF-G concentration on incorporation of prolines in translation
• Genetic code expansion via integration of redundant amino acid assignment by finely tuning tRNA pools
• Efficient cell-free evolution of RNA polymerases by droplet microfluidics
• Boosted cell-free gene expression for robust signal readout from a single-copy DNA template in microdroplets

• 標的細胞選択的に細胞質へmRNAを送達するタンパク質カプセルの開発
• 酵素発現量を自律的に最適化する代謝経路の構築
• リボソームトンネルの改変によるペプチドへの多数のＤ－アミノ酸の翻訳導入手法の開発

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