University of Shizuoka · Graduate School of Pharmaceutical Sciences / Faculty of Pharmaceutical Sciences · 准教授

Research keywordsreverse transcriptase fidelity・RNase H2 mutations・wheat beta-amylase・recombinase polymerase amplification・enzyme engineering・STAT3 inhibitors・protein stability・Aicardi-Goutieres syndrome

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• Solvent engineering studies on recombinase polymerase amplification
• Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA.
• Recombinase polymerase amplification using novel thermostable strand-displacing DNA polymerases from Aeribacillus pallidus and Geobacillus zalihae.
• The cleavage site preference of the porcine pepsin on the N-terminal α1 chain of bovine type I collagen: a focal analysis with mass spectrometry.

• 昆虫種特異性の高い成長制御剤開発に向けたPTTHとそのレセプターの相互作用の解明
• 温度勾配ゲル電気泳動によるHIV-1逆転写酵素遺伝子変異の迅速検出
• 高機能等温核酸増幅酵素の創製と構造解析および病原体の現場での迅速検出への応用
• 耐熱型逆転写酵素の耐熱化機構の解明

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