The University of Shiga Prefecture · Faculty of Environmental Science · 講師

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• Adaptive evolution of sesquiterpene deoxyphomenone in mycoparasitism by Hansfordia pulvinata associated with horizontal gene transfer from Aspergillus species2025 · DOI： 10.1128/mbio.04007-24
• Development of a gene-targeting system using CRISPR/Cas9 and utilization of pyrG as a novel selectable marker in Lentinula edodes2023 · 出版者・発行元： FEMS Microbiology Letters First, we attempted to recombine the Shiitake (Lentinula edodes) pyrG (ura3) gene homologously by introducing a donor vector containing a carboxin resistance gene (lecbxR) flanked by homologous sequences of pyrG into protoplasts of the fungus. However, all the carboxin-resistant transformants only contained ectopic insertions of the exogenous gene and no homologous insertions. Agaricomycetes are generally known for their low efficiency of homologous recombination, and a similar result was shown for L. edodes. We then co-introduced a Cas9 plasmid vector containing a CRISPR/Cas9 expression cassette targeting pyrG and donor plasmid vector. As a result, ΔpyrG strains containing the expected homologous recombination were obtained. However, only two of the seven ΔpyrG strains had the Cas9 sequence; the others did not. Our results suggest that genome editing occurred via the transient expression of the CRISPR/Cas9 cassette in the Cas9 plasmid vector introduced into the fungal cell. Transforming pyrG into a ΔpyrG strain (strain I8) resulted in prototrophic strains with an efficiency of 6.5 strains/experiment. DOI： 10.1093/femsle/fnad042 Scopus
• An exocyst component, Sec5, is essential for ascospore formation in Bipolaris maydis .2021 · 記述言語： 英語 掲載種別： 研究論文（学術雑誌） 参加形態： 共同（副担当） DOI： 10.47371/mycosci.2021.05.002
• A serine/threonine kinase gene BcATG1 is involved in conidiation and sclerotial development in Botrytis cinerea

• 植物病原菌におけるユビキチン・プロテアソーム系を介した病原性制御機構の解明
• 植物病原菌の病原性に関与するプログラム細胞死機構の解明
• 植物病原菌に寄生する菌寄生菌のキラートキシンの機能解明

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