Utsunomiya University · Faculty of Agriculture · 准教授

Research keywords応用微生物学・二次代謝・アミノ酸生合成酵素・ストレス耐性・放線菌・生合成・酵母・大腸菌

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• Bread dough is rich in polysaccharides and polyphenols, which hinder RNA extraction. We therefore established a streamlined workflow combining Fruit-mate homogenization, clarification, TRIzol extraction, high-salt precipitation, and activated-charcoal cleanup. This protocol reproducibly yields intact RNA of sufficient purity, enabling transcriptomic analyses of yeast activity in complex dough environments.論文
• Shota Isogai,Akira Nishimura,Hiroshi Takagi論文
• Characterization of a new Saccharomyces cerevisiae isolated from banana stems and its mutant with l-leucine accumulation for awamori brewing.論文
• Evaluation of synthetic peptides for direct protein delivery into Saccharomyces cerevisiae

• 酵母のスレオニン脱アミノ化酵素に見出した新規活性制御機構の解明2023 · 研究課題/領域番号： 23K05040 2023年4月 - 2026年3月

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